Phospho-slp-76 ser376

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August undefined, 2024

WebPhospho-SLP-76 (Ser376) control lysate. This lysate is a component of the phospho-SLP-76 (Ser376) kit. It may be used as a residue specific positive control for SLP-76 phosphorylation. Prices may differ between countries.

SLP76 ELISA Kits Biocompare

Webhuman phospho-SLP-76 (Ser376) as compared to kit #30794. Background: SH2 domain-containing leukocyte protein of 76 kDa (SLP-76) is a hematopoietic adapter protein that is … WebJan 20, 2024 · We report here that SLP76-S376A-expressing mice exhibit normal thymocyte development and no detectable phenotypic alterations in mature T cell subsets or other lymphoid and myeloid cell lineages. Biochemical analyses revealed that mutant T cells were hypersensitive to TCR stimulation. sm definition slang

AlphaLISA SureFire Ultra p-SLP-76 (Ser376) HV Assay Kit, …

Web(14745) Phospho-SLP-76 (Ser376) (D9D6E) Rabbit mAb Supplier Cell Signaling Technology. Host Rabbit Type Primary Clonality Recombinant (D9D6E) Recombinant Yes Conjugate Unconjugated Target Lymphocyte cytosolic protein 2 (Human) UniProt Q13094 - LCP2_HUMAN. Gene LCP2 Modification WebAs Phospho SLP76 (Ser396) is a readout of T cell activation, it was used to monitor the inhibitory effect of PD-L1 recombinant protein on T cell activation. This assay was performed using the two-plate assay protocol. 400 000 Jurkat cells were plated in 25 µL of complete RPMI culture medium with 10% FBS. WebSLP-76 can be phosphorylated on multiple tyrosine residues by the upstream kinases ZAP-70 and Lck. SLP-76 phosphorylation plays an important role in T cell-mediated IL-2 production by allowing phosphorylated Vav to bind; this complex (SLP-76/Vav) stimulates NF-AT and IL-2 gene activation after TCR engagement. high waisted straight jeans outfit

Alpha SureFire® Ultra™ Multiplex - PerkinElmer

Total SLP-76 HTRF kit I Cisbio - PerkinElmer

WebSLP-76 phosphorylated at Ser376 and another that recognizes an invariant epitope of SLP-76. ... Eu-Ab1 and FR-Ab2 (5 µL) for detection of phospho-SLP-76 (S376). • The plate was incubated at RT for . 18 hours. and the TR-FRET signal . was recorded at 665 and 615 nm (EnVision®; lamp excitation). WebSLP76 is a cytosolic adapter protein that functions as a substrate in the T cell receptor (TCR) activation pathway. Upon TCR engagement, SLP76 is phosphorylated on multiple residues, resulting in the protein being … sm department store careersWebtion of SLP-76 at Ser376, but does not affect the level of total SLP-76 protein. The relationship between lysate protein concentration from untreated and H 2 O -treated Jurkat cells and the absorbance at 450 nm using the FastScan™ Phospho-SLP-76 (Ser376) ELISA Kit #30794 is shown in the upper figure. The corresponding western blots using sm davao online shopping

"WebSLP-76 was originally identified as a substrate of the ZAP-70 protein tyrosine kinase following T cell receptor (TCR) ligation in the leukemic T … " - Phospho-slp-76 ser376

Phospho-slp-76 ser376

Web76 kDa (SLP-76) is a hematopoietic adapter protein that is important in multiple biochemical signaling pathways and necessary for T cell development and activation (1). ZAP-70 … WebFeb 27, 2024 · Image: ELISA detection of phospho-SLP-76 (Ser376) using a FastScan™ sandwich ELISA kit from Cell Signaling Technology. Treatment efficacy is supported by Western blot analysis. Consider using automation

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WebJan 1, 2024 · Product Description. Phosphor- and total SLP-76 assays for monitoring T cell receptor signaling Cisbio cell-based homogeneous htrf phospho-slp-76 (Ser376) … WebThis SLP-76 (Phospho-Ser 376) TR-FRET Assay Kit uses a homogeneous TR-FRET assay method amenable to rapid measurement of phosphorylated protein levels in cells. This SLP-76 (Phospho-Ser 376) TR-FRET Assay Kit is robust, with a Z’ value of 0.92, and suitable for screening a large number of samples.

Webimmunosuppressive environment in the presence of prostaglandin E2 (PGE-2) and are able to selectively inhibit phosphorylation of Ser376 of SLP-76. In addition, in vivo anti-tumor … WebPhospho-SLP-76 (Tyr128) Fluorometric Cell-Based ELISA Kit Detection Target: SLP-76 Reactivity: Human, Mouse, Rat Quantity: 2x96 Wells Supplier Page Sign In or Register to …

WebSLP-76 was originally identified as a substrate of the ZAP-70 protein tyrosine kinase following T cell receptor (TCR) ligation in the leukemic T cell line Jurkat. The SLP-76 locus … WebMay 2024 - Sep 20241 year 5 months. 1250 S Collegeville Rd, Collegeville, PA 19426. • Demonstrated the in vivo efficacy of various GSK’s proprietary small molecules for their …

WebThe Phospho-SLP-76 (Tyr145) assay measures SLP-76 when phosphorylated at Ser376. Unlike Western Blot, the assay is entirely plate-based and does not require gels, electrophoresis, or transfer. The Phospho-SLP-76 (Tyr145) assay uses 2 labeled antibodies: one with a donor fluorophore, the other with an acceptor.

WebMitra S, Ghosh B, Gayen N, Roy J, Mandal A. Bipartite Role of Heat Shock Protein 90 (Hsp90) Keeps CRAF Kinase Poised for Activation. J Biol Chem. 2016;291:24579-24593 pubmed high waisted straight leg ankle jeansWebPhospho-SLP-76 (Ser376) Antibody recognizes endogenous levels of SLP-76 protein only when phosphorylated at Ser376. This antibody cross-reacts with a 145 kDa protein of … high waisted straight leg cargo pantsWebNo-wash kit for detection and quantitation of phospho-SLP-76 in cells using AlphaLISA SureFire technology. We are now two standalone organizations Life Sciences & … sm data sheetWebThis SLP-76 (Phospho-Ser376) TR-FRET Assay Kit uses a homogeneous TR-FRET assay method amenable to rapid measurement of phosphorylated protein levels in cells. This SLP-76 (Phospho-Ser376) TR-FRET Assay Kit is robust, with a Z’ value of 0.92, and suitable for screening a large number of samples. sm department store fairviewWebThe mouse phospho-SLP-76 (Ser376) kit enables the cell-based quantitative detection of mouse phosphorylated SLP-76 at Ser376, for monitoring T-lymphocyte activation. See … high waisted straight leg cropped jeansWebLymphocyte cytosolic protein (SLP-76) is required for T-cell activation and has been implicated in a number of signaling pathways. SLP76 is phosphorylated at Ser376 by HPK1, and this phosphorylation leads to a decrease in T-cell receptor signaling. o Description of the LANCE Ultra Assay sm dictionary\u0027sWebThe Mouse Total SLP-76 cellular assay kit is designed to monitor the expression level of SLP-76, whether phosphorylated or unphosphorylated. It is compatible with our Mouse phospho-SLP-76 (Ser376) kit, and enables the analysis of phosphorylated and total proteins from a single sample for better readouts of T-lymphocyte activation. Benefits high waisted straight leg black jeans