Lineweaver-burk plots for trypsin inhibitor
Nettet27. des. 2016 · ( A) Lineweaver-Burk plot of PTP1B inhibition of alaternin was analyzed in the presence of different concentration of sample as follows: 0 µM ( ), 0.8 µM ( ) and 4.0 µM ( ) of alaternin. ( B) Dixon plots of PTP1B inhibition by alaternin: 1 mM ( ); 0.5 mM ( ) and 0.25 mM ( ) of pNPP. Nettet29. mai 2007 · Lineweaver-Burk plot for the relationship between the rate of chlorination and H 2 O 2 concentration. The reaction mixture contains 300 μmoles of PBS, pH 2.75; 60 μmoles KCl; 0.6~3.0 μmoles H 2 O 2; 0.3 μmole monochlorodimedon; 0.1 μM Fe porphyrin-MAb 1F2 (1:1) in a total volume of 3 ml. Table 1. Kinetic constants of abzyme …
Lineweaver-burk plots for trypsin inhibitor
Did you know?
NettetThe activity against trypsin was inhibited by Co 2+, Mn 2+, Fe 3+, Al 3+, and epoxy chloropropane. Inhibition kinetic assay of SKTI against trypsin as Lineweaver-Burk … Nettet10. okt. 2024 · Because excessive melanin accumulation in the skin can induce hyperpigmentation, the development of tyrosinase inhibitors has attracted attention in …
NettetAnalysis of the Lineweaver–Burk plot demonstrated that these peptides served as non-competitive ACE inhibitors. Molecular docking study showed that these peptides could … NettetI have plotted the lineweaver burk plot with 4 concentrations of inhibitor (0 uM, 125 uM, 250 uM and 500 uM). I have a few questions as below : 1) When we make the enzyme …
NettetThe Lineweaver-Burk plot derives from a transformation of the Michaelis-Menten equation , in which the rate is a function of the substrate concentration and two parameters , the limiting rate, and , the Michaelis constant. Taking reciprocals of both sides of this equation it becomes as follows: Nettet1. sep. 2024 · When used for determining the type of enzyme inhibition, the Lineweaver–Burk plot can distinguish competitive, non-competitive and uncompetitive inhibitors. Competitive inhibitors have the same y-intercept as …
Nettet14. okt. 2016 · Michaelis constant ( Km) and the maximum velocity ( Vmax) values were determined by means of Lineweaver-Burk plots, using initial velocities obtained over a substrate concentration ranging from 250, 500, and 750 nM. Graphs were plotted by using Sigma plot software (Version 12.0, Systat software Inc., San Jose, CA, USA). 2.4.
Nettet14. okt. 2016 · As shown in Figure 2, the line for various concentrations of the substrates in the Dixon plots and those for biochanin A in the Lineweaver–Burk plot showed the … tryfeetNettetLineweaver-burk plot of ACE inhibition by different concentrations of pepsin (A), trypsin (B) and Alcalase (C) NSPHs at varying FAPGG concentrations. Source publication +1 … tryfer terrace harlechNettetIn biochemistry, an Eadie–Hofstee plot (or Eadie–Hofstee diagram) is a graphical representation of the Michaelis–Menten equation in enzyme kinetics.It has been known by various different names, including Eadie plot, Hofstee plot and Augustinsson plot.Attribution to Woolf is often omitted, because although Haldane and Stern credited … philip wade blickNettet1. Types of inhibitors according to lineweaver-burk plot. Lineweaver–Burk plots of (a) competitive inhibition; (b) uncompetitive inhibition; (c) noncompetitive inhibition. 2. … try-featherNettetAnalyzing the generated Lineweaver-Burk diagrams, we observed that LsCTI was a noncompetitive inhibitor for both chymotrypsin ( Figure 4 A) and trypsin ( Figure 4 B) … tryfeminorNettetLineweaver-Burk Plot for enzyme inhibition. Contributed by Bizz1111 CC0 1.0 Universal (CC0 1.0) Public Domain Dedication … tryfertr.6cdkfNettetLineweaver-Burk and Dixon plots and their secondary replots indicated that all four compounds possessed non-competitive inhibition. The Ki values of hesperetin, rutin, … tryferotita