WebAssay A. Cylinder plate or cup plate method • A previously liquefied medium with the required quantity of microbial suspension is inoculated • The suspension is added to the medium at a temperature between 40-50 degree and … WebDec 17, 2012 · Two general methods are usually employed, the cylinder-plate (or cup-plate) method and the turbidimetric (or tube assay) method. The cylinder-plate method (Method A) depends upon diffusion of the …
Microbiological assay of antibiotics - SlideShare
WebAgar nutrient content, cylinder charge volume, thickness (volume) of the agar layer, and incubation temperature were 4 factors varied to determine their effect(s) on the optimization of the cylinder-plate diffusion assay. Chlortetracycline was the pilot antibiotic and Bacillus cereus was used as the … WebThe present work reports a microbiological assay based on the cylinder-plate method for determination of clavulanic acid. The assay is based on the inhibitory effect of clavulanic … r/dwphelp
PLA 3.0 – Software for Biostatistical Analysis: Cylinder-plate assays ...
WebThe microbiological assay of an antibiotic is based upon a comparison of the inhibition of growth of microorganisms by measured concentrations of the antibiotics under examination with that produced by known concentration of a standard preparation of the antibiotic having a known activity. WebThe basic materials required for cylinder-plate testing are disposable Petri dishes with lids and 8-10 millimeters (mm) high porcelain cylinders. A stock solution with a USP Reference Standard of a given antibiotic is prepared in the concentrations specified in Table 2 (reproduced from USP 81). WebJun 23, 2024 · The concentrations of these compounds were evaluated by cup-plate technique using suitable test organisms (Staphylococcus aureus ATCC 29737, Staphylococcus epidermidis ATCC12228, Micrococcus ... how to sow turnip greens