Cdh5-creert2-tdtomato
WebApr 22, 2024 · (F) Immunofluorescence on E14.5 placenta from Cdh5 CreERT2 Rosa26 tdTomato embryos with E7.5 OHT using antibodies to PECAM-1 and F4/80. (G) Pulse-labeling of E12.5, E14.5 and E16.5 placenta macrophages from Cdh5 CreERT2 Rosa26 YFP embryos with E10.5 OHT. WebJan 12, 2024 · a, Cdh5 CreERT2;R26 tdTomato construct of the double transgenic mice used for 3D surface rendering of adult aortae to visualize the spatial location of intimal CD45 + cells (green) relative to the ...
Cdh5-creert2-tdtomato
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WebFeb 17, 2024 · strain: Cdh5-Cre;LSL-tdTomato disease state: glioblastoma (GBM) tissue: GBM tumor: Treatment protocol: GBM was induced by orthotopic injection in Cdh5 … WebArticle Snippet: The possibility of Cre-mediated recombination in hematopoietic cells in Cdh5-CreERT2 and PDGFβ-CreERT2 mice was examined with a Rosa26-flox-stop-flox-TdTomato (TdTom) Cre reporter mouse (Jackson Labs #007914). Techniques: Injection, Fluorescence, Two Tailed Test, Immunofluorescence
WebNov 19, 2024 · The Cdh5-CreER, R26R-tdT (tdTomato), Cdh5-DreER, R26R-rox-tdT, UBC-CreER, NR1, Col1a2-CreER, ... cells in the early developing heart contribute to … WebSep 24, 2024 · To achieve induction of Cre gene, female Cdh5-CreERT2-TdTomato mice were gavaged with 400mg/kg of tamoxifen, followed by a two week wash out period. Extracted molecule: total RNA: Extraction protocol: Endothelial mouse lung cells were isolated as previously described (Fehrenbach et al, Am J Physiol Lung Cell Mol Physiol …
WebIn inducible Pdgfb-iCreERT2;mT/mG mice injected with 5 mg tamoxifen and Cdh5(PAC)-CreERT2;mT/mG mice injected with 10 mg tamoxifen, 1 and 2 months after tamoxifen injection, all hematopoietic cells, including megakaryocytes (Fig. 4A, empty arrowheads) expressed tdTomato, suggesting that no hematopoietic expression of the Cre had … WebPlasmid CDH5-AbR-GFP from Dr. Budd A. Tucker's lab contains the inserts CDH5p, CopGFP, and Zeocin and is published in Unpublished This plasmid is available through …
Web033052 B6;129- Procr tm1(cre/ERT2)Yaz /J Procr CreERT2-IRES-tdTomato are knockin/knockout mice with an inducible cre /ERT2 fusion gene followed by a tdTomato …
WebDec 1, 2006 · Additionally, we used an inducible endothelial lineage tracing system of Ind.Endotrack (Cdh5-CreER T2 -TdTomato) mice 20, 21, in which TdTomato is constitutively expressed after Tamoxifen ... gutter deicing cable reviewsWebFig. 4 Detection of tdTomato (red) in various tissues of Cdh5 CreERT2/+; Rosa26 tdTomato/+ mice. CreERT2 mediated recombination can be detected in the brain, pituitary gland, eyes, heart, lung, liver, submandibular gland, kidney, pancreas, testis, muscle, intestine, colon, spleen, thymus and brown fat. TdTomato expression can not be … box working spaceWebIn our final set of lineage tracing experiments, we used the Cdh5-tdTomato mouse line to investigate the potential contribution of endothelial-to-mesenchymal transition (EndoMT) to pulmonary vascular remodeling. In contrast to other endothelial CreERT2 mouse lines such as Tie2, Cdh5-tdTomato does not show labeling of bone marrow-derived cells 22. boxwork textureWebThe lungs from PBS-perfused mice (tdTomato fl/fl Cdh5-CreERT2) were harvested and fixed in 4% paraformaldehyde in 4°C for 24 hours. The lung tissues were then embedded in paraffin. The paraffin slides were acquired by sectioning the fixed and embedded lung tissues. For fresh frozen sections, perfused lung tissues were harvested from C57BL/6J ... box work showsWebView Tg(Cdh5-cre/ERT2)1Rha allele: origin, molecular description and human disease models, images, gene associations, and references. box workspaceWebCdh5(PAC)-CreERT2 with well-characterized Tie2-Cre mice. Results: Adult Cdh5(PAC)-CreERT2 mice are endothelial specific but require a dose of 10 mg of tamox-ifen to allow constant Cre expression. Pdgfb-iCreERT2 mice injected with 5 mg of tamoxifen are appropriate for most endothelial research fields except liver studies, as hep- box world bankWebNov 15, 2024 · To induce PAH in Cdh5-CreERT2-TdTomato mice and C57/BL6 mice, female mice were treated with 3 weeks of weekly 20 mg/kg SU5416 injection, while exposed to chronic hypoxia (10% oxygen) as previously described. 24, 25 At the end of the procedure, RVSP was measured under terminal anaesthesia (4% isoflurane) and the … boxwork wind cave