Web(FBS; BioInd, Israel) at 37°C in humid air with 5% CO2. Exosomal extraction Exosomes were extracted and purified as previously de-scribed [26]. The protein content of isolated exosomes was quantified using a BCA protein assay kit (CWBIO, Beijing, China). The concentration of hucMSC-derived exosomes for in vitro use was 200μgml−1 and a total of WebIsrael +9724-996-0595. bioind.com. Contact. Profile. Contact. About Biological Industries. BIOLOGICAL INDUSTRIES (BI) develops, manufactures and supplies life science products to universities, government research and healthcare institutions and to the bio-pharmaceutical industry. With an in-house R&D Department and over 30 years of …
The anti-tumor growth effect of a novel agent DMAMCL in ...
WebApr 7, 2024 · Neonatal rat cardiomyocytes were isolated from the ventricular myocardium of 1- to 3-day-old SD rats by enzymatic digestion as previously described and cultured in DMEM (Corning, USA) with 10% horse serum (Gibco, Grand Island, USA) and 5% fetal bovine serum (FBS, BioInd, Israel). All assays and transfections on NRCM were … WebApr 26, 2024 · Glioma cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM, Bioind, Israel) containing 10% fetal bovine serum (Bioind, Israel) and 1% penicillin/streptomycin (Gibco, USA) at 37°C with 5% CO 2. Sh-SY5Y cells were cultured in DMEM/F12 (Gibco, USA). Protein Extraction and Western Blotting herring landing jefferson wi
hucMSC-derived exosomes attenuate colitis by regulating …
WebJan 20, 2024 · Israel’s Biond Biologics to get $125 million from Sanofi to develop cancer drug Under the terms of license agreement, Biond could get $1 billion as milestones met; … WebJan 27, 2024 · HGC-27, MKN-45, GES-1 cells were cultured in RPMI 1640 (Bioind, Israel)). MGC-803 and HEK-293 T cells were cultured in high glucose-DMEM (Bioind, Israel) and AGS was cultured in DMEM/F-12 (Bioind, Israel). All cell lines were maintained in DMEM supplemented with 10% fetal bovine serum ... WebMar 31, 2024 · The cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Gibco, USA) supplemented with penicillin (400 U/ml), streptomycin (50 mg/ml), and 10% fetal bovine serum (FBS, Bioind, Israel) at 37 °C in a 5% CO 2 environment. HaCaT cells were incubated with or without 50 ng/ml TNF-α and/or 50 nM GW for 12 or 48 h. may 23 birthday horoscope